|
KMID : 0545119940040030200
|
|
Journal of Microbiology and Biotechnology
1994 Volume.4 No. 3 p.200 ~ p.203
|
|
|
Insect Cell Culture for Recombinant β-galactosidase Production Using a Spin-filter Bioreactor
|
|
Kim, Hak Ryul
Lee, Ki Woong/Kim, Tae Yong/Oh, Jai Hyun/Yang, Jai Myung/Kang, Seok Kwon/Chung, In Sik
|
|
|
Abstract
|
|
|
|
Spodoptera frugiperda IPLB-SF-21-AE cells were cultivated in a spin-filter bioreactor with continuous perfusion for the recombinant β-galactosidase production. At the perfusion rate of 0.06 hr^-1, the maximum cell density of insect cells in this bioreactor system reached 3.5×10 exp (6) viable cells/㎖ using the Grace media containing 5% FBS and 0.3% Pluronic F-68. The recombinant β-galactosidase production of 8,100 units per reactor volume was also achieved at this perfusion rate.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|